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Hematoxylin and eosin staining of tissue and cell sections

The hematoxylin and eosin (H&E) stained tissue section is the cornerstone of anatomical pathology diagnosis. The H&E procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components. However, staining results are dependent on proper specimen processing, which involves tissue preservation, dehydration, clearing, and paraffin infiltration. While improvements in instrumentation for both tissue processing and staining have been beneficial, limitations in the chemical reagents used must always be considered.X.-J. Yang, Y.-W. Zheng, L.-Q. Zheng, L.-Z. Wu, C.-H. Tung and B. Chen, Green Chem., 2019, 21, 1401–1405 RSC. a) H. Wang, Y. Li, Z. Tang, S. Wang, H. Zhang, H. Cong and A. Lei, ACS Catal., 2018, 8, 10599–10605 CrossRef CAS; d) M. Majek, F. Filace and A. Jacobi von Wangelin, Beilstein J. Org. Chem., 2014, 10, 981–989 CrossRef PubMed ; For a useful review on metal free C–X bond formation containing many eosin-mediated reactions, see:;

Éosinophilie - Hématologie et oncologie - Édition

Excited state eosin initially acting as an oxidant ( *EY → EY˙ −) in cyclization reactions. With cyclic compounds making up the vast majority of target compounds for synthesis, new ways to form rings are always of value. G. Zhang, L. Zhang, H. Yi, Y. Luo, X. Qi, C.-H. Tung, L.-Z. Wu and A. Lei, Chem. Commun., 2016, 52, 10407–10410 RSC.

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